B983 

CHLORELLA BROTH WITHOUT DEXTROSE AND CITRATE

Formula

Ingredients 

gms/lit.

Cupric sulphate

Sodium molybdate 

Zinc sulphate 

Boric acid 

Manganese sulphate

Ferrous sulphate

Potassium sulphate 

Magnesium sulphate

Monopotassium phosphate 

Potassium nitrate

0.0000078

0.00005

0.00022

0.00028

0.0014

0.0015

0.217

2.40

2.45

2.50

Final pH (at 25°C) : 4.5 + 0.2

Directions 

Suspend 7.6 gms. in 900 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Aseptically add 100 ml of separately sterilized solution containing 10 gms. of dextrose and 32 mg of potassium citrate. Mix well and dispense as desired.

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