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B974 |
CASMAN BROTH BASE |
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Formula |
B974 |
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Ingredients |
gms/lit. |
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Proteose peptone Tryptose Beef extract Dextrose Corn starch Sodium chloride Nicotinamide p-Amino benzoic acid (PABA) |
10.00 10.00 3.00 0.50 1.00 5.00 0.05 0.05 |
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Final pH (at 25°C) : |
7.2 + 0.2 |
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Directions |
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Suspend 29.6 gms. of B974 in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 0.15% v/v sterile waterlysed blood (water:blood : : 3:1) of 5% sterile blood. Mix well and dispense as desired. |
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