B962 

CAFFEIC ACID FERRIC CITRATE TEST AGAR

Formula

Ingredients 

gms/lit.

Yeast extract

Dextrose 

Ammonium sulphate 

Dipotassium phosphate 

Magnesium sulphate 

Caffeic acid 

Ferric citrate 

Agar 

2.00

5.00

5.00

0.80

0.70

0.18

0.02

20.00

Final pH (at 25°C) : 6.5 + 0.2

Directions 

Suspend 33.7 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Dispense and sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 50-55°C. Add 50 mcg/ml of chloramphenicol if desired.

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