B957 

BUSHNELL HASS BROTH

Formula 

B957

Ingredients 

gms/lit.

Magnesium sulphate

Calcium chloride 

Monopotassium phosphate 

Dipotassium phosphate 

Ammonium nitrate 

Ferric chloride 

0.20

0.02

1.00

1.00

1.00

0.05

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 3.27 gms. of B957 in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. A white precipitate prior to sterilization becoming yellow to orange after sterilization is normal. 

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