B894

LISTERIA ENRICHMENT BROTH (TWIN PACK)

Formula 

B894 

Ingredients 

gms/lit. 

Part A :

Casein enzymic hydrolysate 

Peptic digest of animal tissue 

Dextrose 

Sodium chloride 

Thiaminium dichloride 

Acriflavin hydrochloride-(Trypaflavin) 

Part B :

Potassium thiocyanate 

 

10.00 

10.00 

1.00 

5.00 

0.005 

0.01 

 

37.50 

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 26 gms. of Part A  and 37.5gms of Part B in 1000 ml. distilled water. Heat boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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