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B823 |
YEAST CARBON BASE |
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Formula |
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Ingredients |
gms/lit. |
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Dextrose L-Histidine hydrochloride DL-Methionine DL-Tryptophan Biotin Calcium pantothenate Folic acid Inositol Niacin p-Amino benzoic acid Pyridoxine hydrochloride Riboflavin (Vitamin B2) Thiamine hydrochloride Boric acid Copper sulphate Potassium chloride Ferric chloride Manganese sulphate Sodium molybdate Zinc sulphate Monopotassium phosphate Magnesium sulphate Sodium chloride Calcium chloride |
10.00 0.001 0.002 0.002 0.000002 0.0004 0.000002 0.002 0.0004 0.0002 0.0004 0.0002 0.0004 0.0005 0.00004 0.0001 0.0002 0.0004 0.0002 0.0004 1.00 0.50 0.10 0.10 |
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Final pH (at 25°C) : 5.5 + 0.2 |
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Directions |
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For Nitrogen Assimilation test, prepare the broth base in 10X concentration. Dissolve 11.7 gms. in 100 ml. distilled water. Add sterile nitrogen source as desired to it. Warm if necessary to dissolve the medium completely. Sterilization by filtration. For detection of yeasts, other than Saccharomyces cerevisiae, dissolve 2.35 gms. of Yeast Carbon Base in 100 ml. distilled water. For detection and enumeration of wild yeasts in beer and other brewing materials add 0.33 gms. of Ammonium sulphate and 4 gms. of agar to base B. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
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