B785 

TRANSPORT MEDIUM, AMIES W/O CHARCOAL

Formula

Ingredients 

gms/lit.

Sodium chloride

Potassium chloride 

Calcium chloride 

Magnesium chloride 

Monopotassium phosphate 

Disodium phosphate 

Sodium thioglycollate 

Agar 

3.00

0.20

0.10

0.10

0.20

1.15

1.00

4.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 10 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in screw cap bottles or tubes in 6 ml. or desired quantity. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool in an upright position.

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