B772 

TERGITOL – 7 AGAR H

Formula

Ingredients 

gms/lit.

Proteose peptone 

Yeast extract 

Lactose 

Sodium hyptadecyl sulphate 

Bromo thymol blue 

Ferric ammonium citrate 

Sodium thiosulphate
Agar 

5.00

3.00

10.00

0.10

0.025

0.50

 0.50

15.00

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 34.13 gms. in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C

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