B770 

TELLURITE GLYCINE AGAR BASE

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Yeast extract

Mannitol

Dipotassium phosphate

Lithium chloride

Glycine

Agar

10.00

5.00

5.00

5.00

5.00

10.00

16.00

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 56 gms. in 1000ml. distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and to each 100 ml. of base add 2 ml of 1% Potassium Tellurite Solution (BF010). Mix well before pouring into sterile petri plates.

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