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B753I |
MAC CONKEY SORBITOL AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzyme hydrolysate Meat Peptone Sorbitol Bile Salt mixture Sodium chloride Crystal violet Neutral red Agar |
17.00 3.00 10.00 1.50 5.00 0.001 0.03 13.50 |
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Final pH (at 25°C) : 7.1 + 0.2 |
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Directions |
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Suspend 50.00 gms. in 990 ml. distilled water. Heat to boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING. Cool to 40-50 c.and add aseptically 2 vials of Tellurite –Cefaxime supplement Mix well and pour into sterile Petri plates. |
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