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B702 |
RIPPEY-CABELLI AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Tryptose Trehalose Yeast extract Sodium chloride Potassium chloride Magnesium sulphate Iron (III) chloride Bromo thymol blue Agar |
5.00 5.00 2.00 3.00 2.00 0.20 0.10 0.04 15.00 |
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Final pH (at 25°C) : 8.0 + 0.2 |
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Directions |
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Suspend 16.17 gms. in 500ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 5 ml ethanol and rehydrated contents of 1 vial of Rippey-Cabelli Supplement (BF094). Mix well and pour into sterile petri plates |
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