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B684 |
PHENYLALANINE MALONATE BROTH (Shaw & Clarke Medium) |
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Formula |
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Ingredients |
gms/lit. |
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Yeast extract Sodium malonate DL-phenylalanine Ammonium sulphate Dipotassium phosphate Monopotassium phosphate Sodium chloride Bromo thymol blue |
1.00 3.00 2.00 2.00 0.60 0.40 2.00 0.025 |
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Final pH (at 25°C) : 6.3 + 0.2 |
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Directions |
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Suspend 11 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 10 lbs pressure (115°C) for 10 minutes. |
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