B682 

PHENOLPHTHALEIN PHOSPHATE AGAR

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract

Sodium chloride 

Sodium phenolphthalein phosphate 

Agar 

5.00

3.00

5.00

0.012

15.00

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 28 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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