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B665 |
PHENYLETHYL ALCOHOL AGAR |
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Formula |
B665 |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Papaic digest of soyabean meal Sodium chloride Phenylethyl alcohol Agar |
15.00 5.00 5.00 2.50 15.00 |
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Final pH (at 25°C) : 7.3+ 0.2 |
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Directions |
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Suspend 42.5 gms. of B665 in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes. For the preparation of blood agar add 5% v/v sterile defibrinated blood to the sterile molten medium, cooled to 45° C. Mix well and pour into sterile petri plates. |
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