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B653 |
LACTOBACILLUS SELECTION BROTH BASE |
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Formula |
B653 | ||
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Glucose Sodium acetate Monopotasium hydrogen phosphate Ammonium citrate Polysorbate 80 Magnesium sulphate Manganese sulphate Ferrous sulphate Agar |
10.00 5.00 20.00 25.00 6.00 2.00 1.00 0.575 0.12 0.34 --- |
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Final pH (at 25°C) : 5.5 + 0.2 5.4 ± 0.2 |
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Directions |
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Suspend 69.7 gms. of B653 in 1000ml. distilled water containing 1.32ml. glacial acetic acid. Heat with frequent stirring. Boil for 1-2 minutes to dissolve the medium completely. DO NOT AUTO CLAVE. If storage is necessary, autoclave at 12 lbs pressure (118°C) for 15 minutes. |
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