B645 

POTATO DEXTROSE ROSE BENGAL AGAR

Formula

Ingredients 

gms/lit.

Potato infusion from

Dextrose

Rose Bengal

Agar

200.00

20.00

0.0084

15.00

Final pH (at 25°C) : 5.6 + 0.2

Directions 

Suspend 39 gms. in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes Mix well before dispensing. In specific Work when pH 3.5 is required, acidify the medium with sterile 10% Tartaric acid. The amount of acid required for 100ml sterile ,cooled medium is approx. 1ml .DO NOT HEAT the medium after addition of the acid.

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