B604

MacConkey Agar w/Bromothymol blue

Formula

Ingredients

gms/lit

Peptic digest of animal tissue

Proteose peptone

Lactose

Bile salts

Sodium chloride

Bromothymol blue

Agar

17.00

3.00

10.00

1.50

5.00

0.03

15.00

Final pH (at 25°C):7.1 + 0.2

Directions

Suspend 51.53 gms, in 1000 ml. distilled water. Heat to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. 

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