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B601 |
MOF MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Tris hydroxymethyl aminomethane Boric acid Ammonium sulphate Disodium phosphate Ammonium nitrate Sodium chloride Magnesium chloride Sodium sulphate Calcium chloride Potassium chloride Sodium bicarbonate Potassium bromide Strontium chloride Sodium silicate Sodium fluoride Phenol red Agar |
1.00 0.10 0.50 0.011 0.50 0.004 0.0008 9.70 4.40 1.60 0.90 0.275 0.08 0.04 0.017 0.002 0.0012 0.01 3.00 |
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Final pH (at 25°C) : 8.0 + 0.2 |
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Directions |
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Suspend 22.14 gms. in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 55-60°C and aseptically add sterile dextrose solution (or other carbohydrate of choice) to a final concentration of 1%. |
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