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B549 |
GLYCEROL ASPARAGINE AGAR BASE (ISP MEDIUM NO.5) |
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Formula |
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Ingredients |
gms/lit. |
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L-asparagine Dipotassium phosphate Trace salt sodium (ml) Agar |
1.00 1.00 1.00 20.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 23 gms in 1000 ml distilled water containing 10 ml glycerol. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
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