B548 

GLUCOSE YEAST EXTRACT AGAR

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Yeast extract 

Glucose 

Monopotassium phosphate 

Dipotassium phosphate 

Magnesium sulphate

Sodium chloride 

Managanese sulphate 

Zinc sulphate 

Copper sulphate 

Cobalt sulphate 

Agar 

5.00

5.00

2.00

0.50

0.50

0.30

0.01

0.01

0.0016

0.0016

0.0016

15.00

Final pH (at 25°C) : Self

Directions 

Suspend 46 gms in 1000 ml distilled water. Boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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