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B494 |
DEXTROSE SALT BROTH |
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Formula |
B494 |
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Ingredients |
gms/lit. |
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Dextrose Yeast extract Ammonium nitrate Ammonium sulphate Disodium phosphate Monopotassium phosphate Sodium chloride |
10.00 1.00 1.00 1.00 4.00 2.00 1.00 |
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Final pH (at 25°C) : 6.6 + 0.2 |
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Directions |
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Suspend 20 gms. of B494 in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes. Cool to 45°C and add 57ml of sterile 10% aqueous citric acid. Mix well before pouring into sterile petri plates. Do not reheat after addition of citric acid. |
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