B449 

TRANSPORT MEDIUM W/CHARCOAL (CVTR TRANSPORT MEDIUM VIRAL)

Formula

Ingredients 

gms/lit.

Sodium chloride

Potassium chloride

Dipotassium phosphate

Charcoal

Agar

4.00

0.20

1.70

10.00

4.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 20.0 grams in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in screw capped vials as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Invert the vials just before the medium gets to distribute the charcoal uniformly.

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