B428 

PHENOL RED LACTOSE BROTH

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Sodium chloride 

Lactose 

Phenol red 

10.00

5.00

10.00

0.018

Final pH (at 25°C) : 7.5 + 0.2

Directions 

Suspend 25 gms. in 1000 ml. distilled water. Mix well and distribute in tubes with inverted Durham’s tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. 

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