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B366 |
YEAST NITROGEN BASE W/O AMINO ACIDS |
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Formula |
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Ingredients |
gms/lit. |
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Ammonium sulphate Monopotassium phosphate Magnesium sulphate Sodium chloride Calcium chloide Boic acid Coppe sulphate Potassium iodide Ferric chloride Manganese sulphate Sodium molybdate Zinc sulphate Biotin Calcium pantothenate Folic acid Inositol Niacin p-Amino benzoic acid (PABA) Riboflavin (vitamin B2) Pyridoxine hydrochloride Thiamine hydrochloride |
5.00 1.00 0.50 0.10 0.10 0.0005 0.00004 0.0001 0.0002 0.0004 0.0002 0.0004 0.000002 0.0004 0.000002 0.002 0.0004 0.0002 0.0002 0.0004 0.0004 |
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Final pH (at 25°C) : 5.4 + 0.2 |
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Directions |
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For the results the medium is prepared in 10X strength. Suspend 6.7 gms. in 1000ml. distilled water. add 5 gms. dextrose or an equivalent amount of other carbohydrate and other chemicals like amino acids that modify growth of yeasts as desired. Ensure complete solution and sterilize by filtration. For the use, dilute 0.5 ml. 10X medium to make 5 ml. with sterile distilled water. Mix well |
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