|
B364 |
YEAST MORPHOLOGY AGAR |
|
|
Formula |
||
|
Ingredients |
gms/lit. |
|
|
Ammonium sulphate Aspargine Dextrose L-histidine hydrochloride DL-methionine DL-tryptophan Biotin Calcium pantothenate Folic acid Inositol Niacin p-Amino benzoic acid (PABA) Pyridoxine hydrochloride Riboflavin (vitamin B2) Thiamine hydrochloride Boric acid Copper sulphate Potassium iodide Ferric chloride Manganese sulphate Sodium molybdate Zinc sulphate Monopotassium phosphate Magnesium sulphate Sodium chloride Calcium chloride Agar |
3.50 1.50 10.00 0.01 0.02 0.02 0.000002 0.0004 0.000002 0.002 0.0004 0.0002 0.0004 0.0002 0.0004 0.0005 0.00004 0.0001 0.0002 0.0004 0.0002 0.0004 1.00 0.50 0.10 0.10 18.00 |
|
|
Final pH (at 25°C) : 5.6 + 0.2 |
||
|
Directions |
||
|
Suspend 35 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Pour into sterile petri plates to a depth of 1.5 mm. Allow the media surface to dry for one or two days at room temperature. Use light inoculum and make a single streak and two point inoculations near the other sides of the plate. |
||