B331 

THERMOACIDURANS AGAR

Formula

Ingredients 

gms/lit.

Proteose peptone

Yeast extract

Dextrose

Dipotassium phosphate

Magnesium sulphate

Agar

5.00

5.00

5.00

4.00

0.20

20.00

Final pH (at 25°C) : 5.0 + 0.2

Directions 

Suspend 39.2 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and pour into sterile petri plates.

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