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B321 |
SPIROLATE BROTH, OMATA |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Dextrose Yeast extract Sodium chloride Sodium thioglycollate L-Cysteine hydrochloride |
15.00 5.00 5.00 2.50 0.50 1.00 |
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Final pH (at 25°C) : 7.1 + 0.2 |
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Directions |
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Suspend 29 gms. in 1000ml. distilled water. Add 0.25 gms. of TEM-4TR-Diacetyl Tartaric Acid Ester of Monoglycerides of Animal Origin (TEM-4TR) if desired. Heat with frequent stirring and boil for 1 minute. Dispense in test tubes filling them half full (about 15-20 ml in 6” inch tubes.) If bigger containers are used, maintain the surface to volume ratio similar to that of tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool and add sterile inactivated 10% v/v sheep/rabbit bovine serum. |
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