B259 

SEMISOLID NUTRIENT AGAR

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract

Agar

5.00

3.00

4.00

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 12 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Dispense in tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubes to cool in an upright position.

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