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B257 |
MINERAL MODIFIED GLUTAMATE MEDIUM BASE (TWIN PACK) |
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Formula |
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Ingredients |
gms/lit. |
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PART A: Lactose Dipotassium phosphate Sodium formate L-Cystine L-Aspartic acid L(+) Arginine Thiamine Nicotinic acid Pantothenic acid Magnesium sulphate Ferric ammonium citrate Calcium chloride Bromo cresol purple PART B : Sodium glutamate |
20.00 1.80 0.50 0.04 0.048 0.04 0.002 0.002 0.002 0.20 0.02 0.02 12.70
6.35 |
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Final pH (at 25°C) : 6.7 + 0.2 |
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Directions |
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Suspend 22.7 gms. of Part A and 12.7 of Part B in 1000ml distilled water containing 5 grams of ammonium chloride. Heat if necessary to dissolve the medium completely. Dispense in tubes containing in verted Durham’s tubes. Sterilize by autoclaving at 10lbs pressure (115°C) for 10 minutes. For single strength medium use 11.35 gms. of Part A and 6.35 gms. of Part B in 1000ml distilled water containing 2.5 gms. ammonium chloride. |
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