B255

MICRO VITAMIN TEST INOCULUM BROTH

Formula 

B255

Ingredients 

gms/lit.

Yeast extract

Proteose peptone

Dextrose

Monopotassium phosphate

Polysorbate 80

20.00

5.00

10.00

2.00

0.10

Final pH (at 25°C) : 6.7 + 0.2

Directions 

Suspend 37.1 gms. of B255 in 1000ml distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes.

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