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B247 |
MALONATE BROTH, EWING MODIFIED |
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Formula |
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Ingredients |
gms/lit. |
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Yeast extract paste Dextrose Ammonium sulphate Dipotassium phosphate Monopotassiu phosphate Sodium chloride Sodium malonate Bromo thymol blue |
1.00 0.25 2.00 0.60 0.40 2.00 3.00 0.025 |
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Final pH (at 25°C) : 6.7 + 0.2 |
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Directions |
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Dissolve 9.28 grams in 1000 ml. distilled water. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid the addition of carbon and nitrogen from other sources. |
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