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B239 |
SEMI-SOLID RAPPAPORT VASSILIADIS MEDIUM , MODIFIED |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzyme hydrolysate Tryptose Sodium chloride Potassium dihydrogen phosphate Magnesium chloride, anhydrous Malachite green oxalate Agar |
4.59 4.59 7.34 1.47 10.93 0.037 2.70 |
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Final pH (at 25°C) : 5.2 + 0.2 |
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Directions |
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Suspend 15.83 gms. in 500 ml distilled water. Heat to boiling with frequent agitation to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50 C. and aseptically add two vials of Novobiocin supplement(BF083).Mix well and pour into sterile Petri plates. Air dry the plated medium at room temperature for at least one hour. |
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