B223

LETHEEN AGAR (AOAC)

Formula 

B223 

Ingredients 

gms/lit. 

Casein enzymic hydrolysate 

Beef extract 

Dextrose 

Polysorbate 80 

Lecithin 

Agar 

5.00 

3.00

1.00 

7.00 

1.00

15.00 

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 32 gms. of B223 in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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