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B221 |
LEGIONELLA AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Yeast extract Charcoal activated ACES buffer α - Ketoglutarate Potassium hydroxide Agar |
10.00 1.50 6.00 1.00 1.50 17.00 |
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Final pH (at 25°C) : 6.9 + 0.2 |
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Directions |
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Suspend 18.5 gms. in 500ml. distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Do not heat prior to sterilization. Cool to 50°C and aseptically add rehydrated contents of 1 vial of Legionella Growth Supplement (BF115) and Legionella Selective Supplement (BF022). Mix well and pour into sterile petri plates. Stir the medium during dispensing to prevent settling of charcoal particles. |
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