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B214 |
KOHN TWO TUBE MEDIUM NO.2 |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Casein enzymic hydrolysate Sucrose Salicin Sodium chloride Sodium thiosulphate Disodium hydrogen orthophosphate Bromo thymol blue Agar |
10.00 10.00 10.00 10.00 5.00 0.016 0.09 0.02 3.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 48.13 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes. Cool the tubed medium in an upright position. |
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