B198 

GIOLITTI CANTONI BROTH BASE

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate 

Beef extract

Yeast extract 

Mannitol 

Sodium chloride 

Lithium chloride 

Glycine 

Sodium pyruvate 

10.00

5.00

5.00

20.00

5.00

5.00

1.20

3.00

Final pH (at 25°C) : 6.9 + 0.2

Directions 

Suspend 54.2 gms in 1000 ml distilled water. Warm gently to dissolve the medium completely. Dispense 19 ml amounts in 20 mm X 200 mm test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool rapidly to room temperature and aseptically add 0.3 ml of 3.5% Potassium Tellurite Solution (BF008) to each tube. Add 0.03 ml for testing meat and products. Mix well before use.

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