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B177 |
EC BROTH |
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Formula |
B177 |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Lactose Bile Salt mixture Dipotassium phosphate Monopotassium phosphate Sodium chloride |
20.00 5.00 1.50 4.00 1.50 5.00 |
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Final pH (at 25°C) : |
6.9 + 0.2 |
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Directions |
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Suspend 37 gms. in 1000 ml. distilled water. Heat if necessary to dissolve the medium Completely. Dispense in test tubes containing Inverted Durham’s tube. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Adjust the concentration of medium in accordance with sample size. |
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