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B176 |
DUBOS BROTH BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate L-Aspargine Polysorbate 80 Monopotassium phosphate Disodium phosphate Ferric ammonium citrate Magnesium sulphate Calcium chloride Zinc sulphate Copper sulphate |
0.50 2.00 0.20 1.00 2.50 0.05 0.01 0.0005 0.0001 0.0001 |
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Final pH (at 25°C) : 6.6 + 0.2 |
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Directions |
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Suspend 1.3 gms. in 180 ml. distilled water containing 10 ml glycerol. Boil if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 20 ml sterile bovine albumin V or sterile serum to each 180 ml of broth base. |
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