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B159 |
D.C.L.S. AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Proteose peptone Beef extract Lactose Sucrose Sodium citrate Sodium thiosulphate Sodium deoxycholate Neutral red Agar |
7.00 3.00 5.00 5.00 10.00 5.00 2.50 0.03 12.00 |
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Final pH (at 25 C) : 7.2 + 0.2 |
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Directions |
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Suspend 49.5 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C and pour about 20 ml of medium into sterile petri plates and allow to dry for about two hours with covers partially removed. |
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