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B1283 |
YEAST AND MOULD BROTH |
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Formula |
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Ingredients |
gms/lit. |
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Yeast extract Malt extract Peptone Dextrose |
3.00 3.00 5.00 10.00 |
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Final pH (at 25°C) : 6.2 + 0.2 |
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Directions |
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Suspend 21 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Autoclave at 15 lbs pressure (121°C) for 15 minutes. If desired, acidify the medium to pH 3.0-4.0 by adding sterile 10% HCl, Tartaric acid or 10% Citric acid. Selective agents, e.g. penicillin (20 units per ml final concentration) may be added to the medium after sterilization using aseptic technique. |
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