|
B1270 |
TRIPLE SUGAR IRON AGAR |
|
|
Formula |
||
|
Ingredients |
gms/lit. |
|
|
Casein enzyme hydrolysate Lactose Sucrose Dextrose Sodium chloride Ferric ammonium sulphate Sodium thiosulphate Ammonium iron(III) citrate Phenol red Agar |
10.00 10.00 10.00 1.00 5.00 0.20 0.20 0.30 0.024 13.00 |
|
|
Final pH (at 25°C) : 7.4 + 0.2 |
||
|
Directions |
||
|
Suspend 59.42 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Mix well and distribute into test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
||