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B1219 |
MUG – E.C. 0157 AGAR, MODIFIED |
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Formula |
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Ingredients |
gms/lit. |
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Peptone from casein Meat extract Yeast extract Sorbitol Ammonium iron (III) citrate 4-Methylumbelliferyl-β-D-glucuronide Sodium chloride Bromothymol blue Sodium thiosulfate Sodium deoxycholate Agar – Agar |
20.00 2.00 1.00 10.00 0.50 0.10 5.00 0.025 2.00 1.12 13.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 55.00 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
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