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B1132 |
LISTERIA IDENTIFICATION AGAR BASE, MODIFIED (PALCAM) |
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Formula |
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Ingredients |
gms/lit. |
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Peptone Yeast extract Sodium Chloride Starch D (-) Mannitol Ammonium ferric citrate Esculin Dextrose Lithium chloride Phenol red Agar |
23.00 3.00 5.00 1.00 10.00 0.50 0.80 0.50 15.00 0.08 13.00 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 71.88 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to around 50°C and aseptically add rehydrated contents 1 vial of Listeria Selective Supplement (PALCAM) (BF072). Mix well and pour into sterile petri plates. |
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