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B1088 |
KLIGLER IRON AGAR MODIFIED |
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Formula |
B1088 |
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Ingredients |
gms/lit. |
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Beef extract Yeast extract Casein enzymic hydrolysate Sodium chloride Lactose Glucose anhydrous Ferrous sulphate Sodium thiosulphate,pentahydrate Phenol red Agar |
3.00 3.00 20.00 5.00 10.00 1.00 0.20 0.30 0.025 15.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 57.3gms. of dehydrated B1088 in 1000 ml. distilled water. Boil to dissolve the medium completely. Dispense in tubes & sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubes to cool in slanted position to form slope of about 1 inch butts. Best reactions are obtained on freshly prepared media. |
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