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B1040 |
FLUCONAZOLE TESTING MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Part B : Dextrose Potassium dihydrogen phosphate Ammonium sulphate L-Glutamine Magnesium sulphate (anhydrous) Sodium chloride Calcium chloride L-Lysine monohydrochloride Valine L-Arginine monohydrochloride L-Histidine DL-Methionine Tryptophan Inositol Boric acid Caclium d-pantothenic acid Nicotinic acid Pyridoxine hydrochloride Aneurine hydrochloride Zinc sulphate p-Amino benzoic acid (PABA) Riboflavin Ferric chloride Cupric sulphate Biotin crystalline Folic acid L-Leucine L-Isoleucine Sodium molybdate Potassium iodide Threonine |
19.98 1.99 4.99 0.58 0.99 0.20 0.20 0.073 0.047 0.042 0.023 0.0189 0.02 0.00397 0.00099 0.00079 0.00079 0.00079 0.00079 0.0014 0.000395 0.000395 0.000395 0.00012 0.000004 0.000395 0.052 0.052 0.00047 0.0002 0.0476 |
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Final pH (at 25°C) : Self |
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Directions |
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Part A : Suspend 2 gms. of Part A (Agar powder) in 100 ml distilled water, add 0.1 ml phosphate buffer to adjust the pH to 7.5.Heat to boiling to dissolve the agar particles completely and then sterilize by autoclaving at 10 lbs pressure (115°C) for 10 minutes. Part B : suspend 29.34 gms. of Part B in 900 ml. Distilled water. Mix well, add 2 gms. of Sodium bicarbonate, after stirring. Sterilize by filtration. The medium can be kept at 4°C for two weeks. Complete medium is prepared by aseptically adding equal volume of molten Part A (previously cooled to 50°C) and Part B. Mix thoroughly and pour into sterile petriplates. |
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