B1028

ENTEROCOCCUS PRESUMPTIVE BROTH

Formula 

B1028 

Ingredients 

gms/lit.

Casein enzymic hydrolysate 

Yeast extract 

Dextrose 

Sodium azide 

Bromothymol blue 

5.00

5.00

5.00

 0.40

0.032

Final pH (at 25°C)

8.4 + 0.2

Directions

Suspend 15.4 gms. of B1028 in 1000 ml. distilled water. Boil to dissolve the medium completely. Dispense in 100 ml quantities in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.  Cool the broth  to room temperature and add 65 units of Penicillin to each 100 ml of broth prior to use.

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