B1018I 

EC BROTH

Formula

Ingredients 

gms/lit.

Tryptose

Lactose 

Bile Salts no.3 

Dipotassium phosphate 

Monopotassium phosphate 

Sodium chloride 

20.00

5.00

1.50

4.00

1.50

5.00

Final pH (at 25°C) : 6.8 + 0.2

Directions 

Suspend 37 gms. in 1000 ml. distilled water. Heat if necessary to dissolve the medium Completely. Dispense in test tubes containing inverted Durham’s tube. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Adjust the concentration of medium in accordance with sample size.

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