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B1016 |
DUBOS OLEIC AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate L-Aspargine Monopotassium phosphate Disodium phosphate Ferric ammonium citrate Magnesium sulphate Calcium chloride Zinc sulphate Copper sulphate Agar |
0.50 1.00 1.00 2.50 0.05 0.01 0.0005 0.0001 0.0001 15.00 |
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Final pH (at 25°C) : 6.6 + 0.2 |
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Directions |
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Suspend 4 gms. in 180 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Aseptically add 20 ml sterile Oleic Albumin Supplement (BF051) and 5,000 to 10,000 units of Penicillin to sterile, cooled 180 ml medium. Mix thoroughly and distribute in sterile tubes or plates. |
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