B1011 

DIHYDROLASE BROTH BASE

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue 

Yeast extract 

Dextrose 

Sodium chloride 

Bromo cresol purple 

5.00

6.00

2.00

30.00

0.032

Final pH (at 25°C) : 6.8 + 0.2

Directions 

Suspend 43 gms. in 1000 ml. distilled water. Heat, if necessary to dissolve the medium completely. Divide in 2 parts . Add 0.5% L-arginine to first portion.Use second portion as control. Dissolve completely and dispense 3.0 ml into 13 mm X 100 mm screw cap tube. Sterilize by autoclaving at 10 lbs pressure (115 C) for 15 minutes.

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