B085 

TELLURITE BLOOD AGAR BASE

Formula

Ingredients 

gms/lit.

Biopeptone

Sodium chloride

Dipotassium hydrogen phosphate

Corn starch

Monopotassium phosphate

Agar

10.00

5.00

4.00

1.00

1.00

10.00

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 31 gms. in 970ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add sterile solution of Haemoglobin (BF036) to a final concentration of 10 mg/L and sterile reconstituted contents of one vial of Vitamino Growth Supplement
(BF037) and 1% Potassium Tellurite (BF010). Mix well and pour into sterile plates or tubes

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